Fowl guinea production thesis

Guinea fowl production - botswana

Guinea fowl production - botswana
1 guinea fowl production jc moreki, phd poultry and rabbits section, division of non-ruminants, department of animal production, private bag 0032, gaborone,.

The expression levels will be used as qtl data to map chromosome regions that control the expression of candidate genes. Their carcass weight and abdominal fat content will be measures. Molecular characterization of the pearl grey guinea fowl through comparative analysis of partial gene sequence constructs. One male and 4-5 females of each chickens and guinea fowl will be mated to create f1 generations. The sequences code for genes such as retinol binding protein, 3-hydroxybutyrate dehydrogenase, and transferring these sequences averaged 685.

About 200 clones of the hypothalamus and pituitary were cloned, cycle sequenced and characterized. Based on the findings, a feasibility study will be conducted to identify the best marketing strategy for the guinea fowl. To meet objective 3, current market status for guinea fowl will be evaluated. Genes that are differentially expressed in adipose tissue between two parental lines will be considered important candidate that regulate growth and fat deposition and their differential expression will analyzed by dnamicroarray. Hence, the objectives of this research were to construct liver, pancreas, small intestine and pancreas cdna libraries for the guinea fowl and sequence five hundred clones from each library an additional objective was to develop chicken and guinea fowl genetic resource populations for comparative mapping of the avian species.

Major genes that control growth and adipose accumulation will be identified by analyzing growth and adipose specific transcriptome in chicken, then testing the influence of these genes on growth and fat content in both chicken and guinea fowl reference populations. Adefope target audiences poultry industry small farmers scientific community extension specialists project modifications nothing significant to report during this reporting period. Journal of poultry science 45249-254 outputs two complementary dna (cdna) libraries for the liver and adipose tissue of the guinea fowl were constructed. Rapid growth rate of meat-type chickens and guinea fowl has compromised carcass quality due to excessive fat deposition, a liability to the processor and consumers. Outputs marker assisted selection is expected to increase accuracy of selection for traits that are difficult-to-measure and least inheritable. Chicken and quail microsatellite markers reveal polymorphisms in guinea fowl. This is part of the effort to determine genes that may be associated with excessive fat deposition in poultry. To meet objective 2, breeder birds (chickens and guinea fowl) will be hatched and reared at tennessee state university poultryresearch farm. Several of the dna sequences were translated into proteins whose open reading frames produced proteins such as phosphatidylinositol glycan anchor biosynthesis, class z nadh dehydrogenase subunit 5 and albumin. Generating novel genomic libraries of the guinea fowl hypothalamus.

Guinea fowl production by indigenous
2005 guinea fowl production by indigenous farmers in zimbabwe h saina, n t kusina , j f kusina, e bhebhe and s lebel department of animal science.

The requirement of these nutrients for optimum growth and production. Strategies for improving growth, production.

Genes that are differentially expressed in adipose tissue between two parental lines will be considered important candidate that regulate growth and fat deposition and their differential expression will analyzed by dnamicroarray. New gene sequences that are unique to the guinea fowl were identified. Farmer participatory research methodology will be used in this project, i. This is part of the effort to determine genes that may be associated with excessive fat deposition in poultry. The f1 half sib families will be crossed to generate f2 reference populations of 250 birds.

Association of research directors symposium abstracts pp 155. The f2 birds will be measure for body weight at 1, 3, 7, 9 weeks of age. Several of the dna sequences were translated into proteins whose open reading frames produced proteins such as phosphatidylinositol glycan anchor biosynthesis, class z nadh dehydrogenase subunit 5 and albumin. To meet objective 2, breeder birds (chickens and guinea fowl) will be hatched and reared at tennessee state university poultryresearch farm. These rna samples will be used for gene expression analysis with microarray and qrt-pcr.

Twenty six cnvs were found in two or more animals. Generating novel genomic libraries of the guinea fowl hypothalamus. Major genes that control growth and adipose accumulation will be identified by analyzing growth and adipose specific transcriptome in chicken, then testing the influence of these genes on growth and fat content in both chicken and guinea fowl reference populations. In addition, complementary dna (cdna) libraries for the hypothalamus, pituitary and pancreas of the guinea fowl were constructed in an effort to evaluate genes that are associted with satiety and fat deposition in ppoultry. The purpose of this study is to improve guinea fowl and chicken carcass quality by selection using comparative genomics. Real time rt-pcr verification of selected genes will be conducted. Messenger rna will be prepared and normalized cdna libraries constructed from the respective tissues. A specific region of the cd8 locus was amplified using the polymerase chain reaction. Inserts will be excised with xhoi and noti, and cloned into pbluescript vector (stratagene). This analysis will help to further understanding of the constraints and opportunities to marketing guinea fowl products intennessee and other states.

Management strategies to improve